Resumo:
Polycyclic aromatic hydrocarbons (PAHs) comprise a large class of organic
compounds, characterized by containing two or more fused aromatic rings in their
structure. The study of this class of compounds is necessary because they are found
in different environments and associated with the degradation of human health,
attributed to a toxic potential, which can lead to mutagenesis and/or carcinogenesis of
different cells. The present project was dedicated to the optimization and validation of
a chromatographic method for the determination of 16 PAHs in atmospheric particulate
matter. Chromatographic conditions were optimized such as: temperature and volume
of injection, carrier gas flow rates, temperature programming of the chromatographic
oven and detection mode (SIM and SCAN). The optimization was performed through
experimental planning with the aid of the Statistica® software, based on the
BoxBehnken factorial planning model. The conditions optimized for the CG / EM
system include: injection temperature (300ºC), injection volume (2.0 µL), split mode
injection (1:10), carrier gas flow rates ((2,0 mL min-1), heating ramp (70ºC/1 min,
heating at
180ºC at a rate of 10ºC min-1 and isotherm of 5 min, new heating at 40ºC at a rate of
5ºC min-1 and isotherm of 5 min, inally heating at 300ºC at a rate of 20ºC min-1 and
isotherm of 3 min) and selective ion detection mode. It has assessed the influence of
each chromatographic parameter on the resolution of the peaks of each HPA.
Validation on the method was done following the parameters required by ANVISA and
INMETRO (CVmédio ˂ 20%), and presented low detection limits (LDnaftaleno=2,8 mg L-1)
and quantification (LQnaftaleno =8,4 mg L-1).
